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dc.contributor.advisorHamouda, Ahmed
dc.contributor.advisorDoherty, Catherine
dc.contributor.advisorAmyes, Sebastian
dc.contributor.authorOpazo, Andres Felipe
dc.date.accessioned2015-04-13T13:32:46Z
dc.date.available2015-04-13T13:32:46Z
dc.date.issued2014-11-28
dc.identifier.urihttp://hdl.handle.net/1842/10024
dc.description.abstractAcinetobacter baumannii is an important microorganism involved in hospital-acquired infections with a remarkable ability to develop resistance to multiple antibiotics (multidrug-resistance, MDR) which makes it a highly troublesome pathogen in many hospitals around the world. Third-generation cephalosporins (such as ceftazidime) and carbapenems (such as imipenem and meropenem) represent important treatment options for infections caused by this microorganism. Nevertheless, the number of strains resistant to these antibiotics has been increasing during the last decade. The ability to capture, mobilise and regulate the expression of resistance-genes of this microorganism is a cornerstone factor in the development of the MDR, where the Mobilome, defined as “all the mobile genetic elements in a cell”, is responsible for its genetic plasticity. The aim of this work was to analyse the role of insertion sequences (ISs), transposon-like structures, resistance-plasmids and ISCR1-like elements in the resistance to carbapenems and ceftazidime in A. baumannii. Fifteen carbapanem-resistant strains of Acinetobacter baumannii isolated from Chile and two ceftazidime-resistant strains from the United Arab Emirates were studied. Different ceftazidime- and carbapenem-resistance genes were analysed and their genetic environments were characterised. The Mobilome in the carbapenem-resistant strains was composed of insertion sequences (ISs), specifically by ISAba1 associated with blaOXA-51-like, ISAba3 associated to blaOXA-58, which in turn was detected in two different plasmids, and ISAba15 interrupting ISAba3. In the case of the ceftazidime-resistant strain, the presence of an ISCR1 element was harbouring the blaPER-7, which was detected in a megaplasmid. The Mobilome, in the strains analysed, was composed of a wide variety of genetic elements, such as plasmids, insertion sequences, ISCR-like elements, which reflects the ability of A. baumannii to use different genetic platforms to capture and use resistance genes, making the Mobilome an important contributor in the resistance and the dissemination of resistance genes among nosocomial pathogens around the world.en
dc.contributor.sponsor
dc.language.isoenen
dc.publisherThe University of Edinburghen
dc.relation.hasversionLopes BS, Gould IM, Opazo AF, Amyes SGB. The resistance profile of Acinetobacter baumannii from the Aberdeen Royal Infirmary. International Journal of Antimicrobial Agents. 2012; 39: 361-362en
dc.relation.hasversionOpazo A, Sonnevend A, Lopes B, Hamouda A, Ghazawi A, Pal T, Amyes SGB. Plasmid-encoded PER-7 β-lactamase responsible for ceftazidime resistance in Acinetobacter baumannii isolated in the United Arab Emirates. Journal of Antimicrobial Chemotherapy. 2012; 67: 1619-22.en
dc.relation.hasversionOpazo A, Dominguez M, Bello H, Amyes SGB, González-Rocha G. OXA-type carbapenemases in Acinetobacter baumannii in South America. Journal of Infection in Developing Countries. 2012; 6: 311 – 316.en
dc.relation.hasversionOpazo A, Vali L, Al Obaid K, Dashti A, Amyes SGB. Novel genetic structure harbouring blaPER-1 in ceftazidime-resistant Acinetobacter baumannii isolated from Kuwait. International Journal of Antimicrobial Agents. 2014;en
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAcinetobacter baumanniien
dc.subjectantibioticsen
dc.subjectnosocomial pathogenen
dc.titleMobilome and antibiotic resistance in Acinetobacter baumanniien
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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