dc.contributor.author | Reid, Robert Armour | en |
dc.date.accessioned | 2016-01-19T16:31:46Z | |
dc.date.available | 2016-01-19T16:31:46Z | |
dc.date.issued | 1965 | |
dc.identifier.uri | http://hdl.handle.net/1842/12855 | |
dc.description.abstract | | en |
dc.description.abstract | This study is an investigation into some aspects of mammalian cell growth
and metabolism in vitro, with particular reference to the actions of uncouplers
of oxidative phosphorylation. Oxidative phosphorylation is the mechanism by
which the cell utilises the energy made available in biological oxidations,
oxidative reactions being linked, or capable of being linked to the phosphorylation
of adenosine diphosphate (ADP) by inorganic phosphate (Pi) according to
the formal equation | en |
dc.description.abstract | AH₂ + B + ADP + Pi ⇌ A + BH₂ + ATP + H₂O | en |
dc.description.abstract | which is the sum of two reactions which are essentially irreversible | en |
dc.description.abstract | AH₂ + B → A + BH₂ (1) | en |
dc.description.abstract | ADP + Pi → ATP + H₂0 (2) | en |
dc.description.abstract | The oxidative reaction (1) must deliver sufficient energy to drive reaction (2)
to the right. Despite its fundamental importance, the me chanism of oxidative
phosphorylation has not yet been described. Since the finding of Loomis and
Lipmann (1948) that 2 :4- Dinitrophenol (2t4 -DNP) uncouples oxidation from respiratory
chain phosphorylations, research into the actions of uncouplers has centred primarily
on three types of biological systems - isolated mitochondria and sub units. tissue
slices, and micro- organisms. To a lesser extent, ascites tumour cells and red
blood cells have been used. With the exception of the work to be described, no
investigation of any length appears to have been carried out on tissue culture cells.
Of these approaches, the most important information on the mechanism of oxidative
phosphorylation and its uncoupling has been derived from investigations of isolated
mitochondria and sub units. As a very large number of reviews provide extensive
coverage of both historical and recent developments (Lardy and Eivehjam, 1945;
Lehninger, 1951, 1955; Chance and Williams, 1956; Slater, 1955, 1958, 1961, 1964;
Lehninger and Wadkins, 1962), it is proposed to outline only the more important
aspects here as an essential background to the work to be described. | en |
dc.publisher | The University of Edinburgh | en |
dc.relation.ispartof | KB thesis scanning project 2015 | en |
dc.title | Growth and metabolism of strain L fibroblasts with reference to the actions of uncouplers of oxidative phosphorylation | en |
dc.type | Thesis or Dissertation | en |
dc.type.qualificationlevel | Doctoral | en |
dc.type.qualificationname | PhD Doctor of Philosophy | en |