Physico-Chemical and analytical studies with particular reference to Vapour-phase Infrared Spectroscopy
The first part of this thesis gives a general introduction to theoretical and practical considerations in the application of infrared spectroscopy to analytical problems. Part 2 gives the results of several investigations made with the aid of vapour-phase infrared methods. Following (a) a brief description of the general experimental procedure, sections describe the results obtained in investigations of (b) the behaviour of thioalkyl compounds under Zeisel reaction conditions (c) the behaviour of propoxyl and butoxyl groups in the Zeisel reaction (d) the Zeisel determination of tert-butyl groups and the anomalous reaction of tert-butyl phenols (e) the determination of small amounts of alcohols in aqueous solution (f) anomalous results arising from cleavage by hydriodic acid (g) the determination of 1,2-diols by a modified Zeisel reaction (h) simultaneous determination of uronic acid and alkoxyl groups in polysaccharides by reflux with hydriodic acid. Part 3 presents the results of a study of the infrared spectra of some carbohydrates in aqueous solution. Several figures indicate the type of spectra which can be obtained. In general the differences between the spectra of simple sugars are not sufficiently distinctive to facilitate the analysis of unknown mixtures of several sugars such as commonly occur in studies of natural products. Part 4 presents a comparison of the results obtainable for the analysis of some of the inorganic components of the ash obtained from natural carbohydrate materials such as plant gums. The methods used included x-ray fluorescence, flame photometry and chemical analysis using ethylene diamine tetraacetate. Comparisons of the results obtained are presented and the advantages and limitations of the methods are discussed.