The methods for estimating the histamine
content of blood and tissue were studied and a
simpler procedure adopted.
The histamine content of normal mice was
determined and it was found that over seventy per
cent of all the histamine in the body was contained
in the skin.
Large doses of histamine were injected
intravenously in mice and the clearance from the
whole animal studied. The time of half clearance
from the whole body was one and a half hours.
From the large part of the dose present in
the skin and gut immediately after injection,it
appeared that histamine was rapidly taken up by the
A part of a large dose of histamine,given to
mice,was excreted in the urine, the faeces
contained little more than was normally present.
Excretion only took place whilst a large
concentration was present in the body.