Abstract
1. Methods of estimation of ribose nucleic acid are
discussed and a reliable method for the estimation
of that substance in isolated cell nuclei described.
2. An inhibitory effect of deoxyribose nucleic acid
on the production of furfural by acid hydrolysis of
ribose nucleic acid is discussed.
3. Methods are given for the isolation of various
types of cell nuclei.
4. Results are Quoted and discussed for the ribose
nucleic acid content of cell nuclei isolated from
normal and regenerating tissues.
5. .the histochemistry of the nucleolus is discussed
in relation to the above results and evidence for
the presence of ribose nucleic acid in the nucleolus
given.
b. A criticism of Brachet's histochemical test for
ribose nucleic acid is given,evidence being produced
to suggest that the current interpretation of the
above test is faulty.
7. The occurrence of ribose nucleic acid in the
nucleolus is postulated.