Cellular regulation of cortisol in vivo by 11-beta hydroxysteroid dehydrogenase type 1
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Date
08/07/2017Author
Anderson, Anna Jane Claire
Metadata
Abstract
Glucocorticoid excess as a result of Cushing’s syndrome or pharmacological
treatment can result in the development of obesity and type 2 diabetes mellitus
(T2DM). The reactivation of cortisone to cortisol is catalysed by 11βHSD1 which is
expressed widely but notably in adipose tissue and liver. Studies have shown
dysregulation of cortisol in these tissues with obesity potentially promoting the
development of T2DM. Inhibition of 11βHSD1 has been attempted as a novel
treatment for T2DM with observed improvement in glycaemic control, body weight
and blood pressure. The efficacy of such agents has been disappointing with few
reaching phase 2 trials. With recent evidence of bidirectional activity of 11βHSD1 in
vivo it becomes apparent that dysregulation may occur at an intracellular rather than
tissue level.
In this thesis I address several key outstanding questions concerning the physiology
and regulation of 11βHSD1 including:
1. Whether combined therapy with metformin alters 11βHSD1 activity and
obscures the efficacy of 11βHSD1inhibitors;
2. Whether the contribution of 11βHSD1 to local cortisol concentrations has
been under-estimated by considering total rather than free cortisol turnover;
and
3. Whether recycling between cortisol and cortisone in adipose tissue and
skeletal muscle in obesity is a neglected feature of 11βHSD1 biochemistry
and function.
Eight obese healthy men with and without type 2 diabetes were recruited to a
randomised placebo controlled cross over trial. They received 4 weeks treatment
with metformin and placebo. Participants with T2DM additionally received
gliclazide as a further control. Using the deuterated tracer D4-cortisol 11βHSD1
activity was measured. Metformin treatment increased whole body 11βHSD1 in both
groups postulated as a result of improved insulin sensitivity.
11βHSD1 is located within cells and so contributes to free tissue cortisol
concentrations but perhaps less so to total (protein-bound) cortisol in plasma. It has
been shown that 11βHSD1 contributes almost half of total circulating cortisol
concentrations at rest. This measurement relied upon blood sampling during steady
state deuterated cortisol (D4-cortisol) infusion with measurements of total (free plus
protein bound) cortisol which may have underestimated true 11βHSD1 activity. This
was therefore investigated by comparing 11βHSD1 activity as calculated using total
compared with free cortisol tracer enrichments. Equilibrium dialysis was performed
separating free from bound portions in plasma samples taken from healthy volunteers
who received D4-cortisol infusion. Analysis revealed similar measurements of
11βHSD1 activity using free compared with total cortisol implicating rapid turnover
of glucocorticoids between the free and bound pools.
On first discovery 11βHSD1 was seen to be a dehydrogenase enzyme in vitro. Later
work recognised reductase activity in vivo and up until recently 11βHSD1 has been
viewed as a predominantly reductase enzyme. As with other enzymes in the same
family, the ability to catalyse both reductase and dehydrogenase depends upon the
availability of substrate and co substrate. Whether dysregulation of 11βHSD1 in the
settings of obesity and T2DM is the result of alteration in directionality at a cellular
level is not known. Firstly bidirectional activity of 11βHSD1 was confirmed in vitro
using HEK-293 cells stably transfected with 11βHSD1. The influence of obesity and
acute perturbation with hyperinsulinaemia was subsequently investigated in vivo in a
random order cross over single blinded case control study involving ten normal
weight and ten obese healthy male volunteers. D4-cortisol and deuterated cortisone
(D2-cortisone) were infused for the measurement of reductase and dehydrogenase
activity of 11βHSD1 respectively with measurements taken across forearm muscle
and abdominal subcutaneous adipose tissue. Across whole body, lean and obese
individuals displayed similar 11β-reductase and 11β-dehydrogenase activity. Across
tissue, 11β-reductase and 11β-dehydrogenase activity was different from zero across
adipose tissue in obese individuals and across skeletal muscle in lean individuals
providing further evidence of tissue specific differences in 11βHSD1 with obesity.
With the addition of hyperinsulinaemia, reductase and dehydrogenase activity was
somewhat increased in lean individuals although there was no statistically significant
difference between lean and obese individuals. Across tissue there was a trend for
obese individuals to display increased 11β-reductase activity across adipose tissue
with hyperinsulinaemia. Comparing the rates of reductase and dehydrogenase
activity revealed predominantly reductase activity across tissue in obese and
dehydrogenase activity in lean individuals. The development of direction specific
inhibitors targeting reductase activity by 11βHSD1 may prove efficacious for the
treatment of obesity.
In conclusion, 11βHSD1 acts as a bidirectional enzyme in vitro and in vivo. Overall
directionality of enzyme activity is altered in a tissue specific manner in the setting
of obesity. We have shown that this intracellular regulation of cortisol is reflected
equally in the metabolically active free pool and total plasma pool. The efficacy of
11βHSD1 inhibitors as novel agents for the treatment of T2DM and coexisting
obesity is not diminished by co-prescription with metformin but may prove more
efficacious through the development of reductase specific inhibitors.