dc.contributor.author | Dickson, Alan James | en |
dc.date.accessioned | 2018-05-14T10:12:23Z | |
dc.date.available | 2018-05-14T10:12:23Z | |
dc.date.issued | 1978 | en |
dc.identifier.uri | http://hdl.handle.net/1842/29730 | |
dc.description.abstract | | en |
dc.description.abstract | Chicken hepatic parenchymal cell suspensions, isolated by an
optimised collagenase digestion, were used for a study of hepatic
glucose metabolism and its control in the chicken. Characterisation of
this iri vitro preparation showed the parenchymal cells immediately after
isolation to be similar to those of whole liver, both morphologically
and metabolically. This similarity suggested that metabolic studies
with isolated hepatocytes might confidently be extrapolated to the
situation in the intact animal. However the preparation quality was
dependent on collagenase contaminants and all preparations exhibited
decreased viability throughout subsequent incubations. | en |
dc.description.abstract | Glycogen metabolism in isolated hepatocyte suspensions
favoured glycogenolysis and under no conditions was net glycogen
synthesis observed. Gluconeogenesis from added precursors was difficult
to discern with fed chicken hepatocytes due to the high basal glucose
production but was readily demonstrated at a constant rate over a two
hour incubation with starved chicken hepatocytes. | en |
dc.description.abstract | The gluconeogenic effectiveness of precursors was generally
similar in isolated hepatocytes and in chickens in vivo. The greater
effectiveness of lactate compared with pyruvate, observed with both
systems (unlike the rat), is probably a consequence of impaired
hydrogen ion transfer during pyruvate gluconeogenesis due to the
mitochondrial location of phosphoenolpyruvate carbcxykinase in the
chicken. Synergistic interactions between substrates were shown to
occur and be important for interpretation of results from isolated
hepatocytes for extrapolation to the situation in vivo. Glycerol was | en |
dc.description.abstract | Physiological concentrations of glucagon stimulated glycogenolysis
and gluconeogenesis from precursors entering the glycolytic pathway above
and below the triose phosphate dehydrogenase step. Although it was
possible to assign a glucagon control point between triose phosphate and
glucose in chicken liver, that between pyruvate and phosphoenol pyruvate
(postulated for the rat) was not observed. | en |
dc.publisher | The University of Edinburgh | en |
dc.relation.ispartof | Annexe Thesis Digitisation Project 2018 Block 18 | en |
dc.relation.isreferencedby | Already catalogued | en |
dc.title | The preparation and properties of isolated chicken hepatocytes | en |
dc.type | Thesis or Dissertation | en |
dc.type.qualificationlevel | Doctoral | en |
dc.type.qualificationname | PhD Doctor of Philosophy | en |