Show simple item record

dc.contributor.authorSchock, Alexandraen
dc.date.accessioned2018-05-14T10:16:05Z
dc.date.available2018-05-14T10:16:05Z
dc.date.issued1996
dc.identifier.urihttp://hdl.handle.net/1842/29987
dc.description.abstracten
dc.description.abstractMalignant catarrhal fever (MCF) is a lethal lymphoproliferative disorder of cattle and deer caused by infection with either Alcelaphine herpesvirus - 1 (AHV-1) or Ovine herpesvirus - 2 (OHV-2). The disease can be transmitted experimentally to rabbits. Despite intensive studies, only very little viral expression can be detected in lesions. It was therefore proposed that the viruses induce an interleukin-2 (IL-2) hyperproduction which is responsible for the lymphoid cell hyperplasia observed. In an initial comparative study, it was shown that all three viruses (AHV-1, OHV-2 and another bovid y-herpesvirus: Hippotragine herpesvirus- 1 (HipHV-1) induced similar hyperplasia of lymphoid organs and accumulations of mononuclear cells in non-lymphoid organs in rabbits. However, the different viruses affected preferentially certain lymphoid organs. It was observed that the lymphoid cells in non lymphoid tissues were CD43+ T-cells which showed evidence of in situ multiplication. A more detailed phenotypic analysis of splenocytes and lymph node cells in AHV-1 infected rabbits showed that the overall proportions of CD43, CD5, CD4, CDS and B-cells were not altered, but that CD5+ and CD8+ cells were significantly enlarged. The hyperplasia of lymph node cells was also investigated by examining the growth characteristics of freshly explanted lymph node cells from AHV-1 infected rabbits. The lymph node cells had a higher thymidine uptake respective to control cultures in the first 24 hours. IL-2 increased viability and thymidine uptake and Con A did not stimulate these cells as expected. Very little IL-2 activity could be detected in supernatants from short term cultures using the CTLL-based bioassay. To establish a RT-PCR/immunoblot for the detection of rabbit EL-2 mRNA, IL-2 cDNA from Con A stimulated rabbit lymphocytes was cloned and partially sequenced. EL-2 transcripts could be detected in lymphoid cells from pyrexic rabbits infected with AHV-1 and in cells from control rabbits. Furthermore, it was shown that lymphoblastoid cell lines (LCL) derived from MCF-affected cattle did not transcribe IL-2. These data lead to the conclusion that DL-2 is involved in the acute state of MCF, but does not have a central role in the pathogenesis.en
dc.description.abstractFarther characterisation of IL-2 dependent LCL showed that they responded weakly to Con A, were inhibited by Cyclosporin A and transcribed constitutively IL-4, IL-10, INFy and TNFa, whereas no EL-1(3 mRNA could be detected. These data together with the results derived from the short term cultures of lymph node cells from AHV1 infected rabbits clearly show that MCF inducing viruses alter the behaviour of lymphoid cells. The possible interference of OHV-2 and AHV-1 with transductional pathways, the expression of IL-2R and the activation of self-reacting lymphocytes is discussed.en
dc.publisherThe University of Edinburghen
dc.relation.ispartofAnnexe Thesis Digitisation Project 2018 Block 18en
dc.relation.isreferencedbyAlready catalogueden
dc.titleCharacterisation of the T-cell proliferation in malignant catarrhal feveren
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


Files in this item

This item appears in the following Collection(s)

Show simple item record