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dc.contributor.authorSelvarajah, Suganyaen
dc.date.accessioned2018-05-14T10:16:07Z
dc.date.available2018-05-14T10:16:07Z
dc.date.issued2001en
dc.identifier.urihttp://hdl.handle.net/1842/29990
dc.description.abstracten
dc.description.abstractMurine gammaherpesvirus (MHV-68) was isolated from a bank vole. It has pathobiological and genetic similarities to other gammaherpesviruses including Herpesvirus saimiri, Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV). It infects laboratory mice and forms plaques on cell monolayers in tissue culture. The pathogenesis and immunology have been widely studied in the mouse system and it has been identified as a useful "small animal model".en
dc.description.abstractMice were infected with 4xl0⁵ PFU MHV-68 intranasally. The virus infects alveolar epithelial cells and causes interstitial pneumonia. In the lungs the virus undergoes lytic replication which is cleared by day 10. Subsequently the virus is found latent in B cells that have been found to be important in the establishment of latency and in the transmission of virus from the lungs to the spleen. The spleen and the lymph nodes at this stage exhibit splenomegaly and lymphadenopathy respectively. In B cell deficient (μMT) mice, latently infected cells are not detected in the spleen and splenomegaly is not observed.en
dc.description.abstractIn this study, the early events following MHV-68 infection in vivo were examined. The work focused on the mediastinal lymph node (MEN) that drains the lungs. Events occurring in the MLNs from the wild type C57BL/6 mice were compared to those of the pMT mice. The results demonstrated the absence of lymphadenopathy in pMT mice and indicated that lymphadenopathy in wild type C57BL/6 mice was brought about especially by the accumulation of B cells. Depletion of CD4+ T cells in wild type C57BL/6 mice demonstrated that the absence of CD4+ T cells only partially affected the accumulation of B cells and enlargement of the MLN. The pMT mouse showed the presence of latent virus in the MLN even in the absence of B cells. However by day 10 these cells were below levels of detection and there was no evidence of systemic transfer of virus.en
dc.publisherThe University of Edinburghen
dc.relation.ispartofAnnexe Thesis Digitisation Project 2018 Block 18en
dc.relation.isreferencedbyAlready catalogueden
dc.titleEarly events following murine gammaherpesvirus (MHV-68) infectionen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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