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Studies on vitamin C

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MeiklejohnGT_1940redux.pdf (10.35Mb)
Date
1940
Author
Meiklejohn, George T.
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Abstract
 
 
The oxidase activity of cucumber juice is proportional to the amount of copper in the juice after all the "free" copper and other extraneous materials have been removed by prolonged dialysis against pure water. This activity is much greater than the catalytic power of copper in the ionic state or copper in loose non-specific combination with protein. Removal of the copper present in cucumber juice after long dialysis against water by further dialysis against cyanide or dilute mineral acid inactivates the oxidase. Oxidase preparations inactivated by dialysis against cyanide are restored to activity by the addition of small excesses of copper sulphate, the restored enzyme showing greater catalytic activity than the amount of copper effecting the restoration. It is concluded that cucumber juice contains a discrete ascorbic acid oxidase, a metal protein in which the prosthetic group is copper and which is not identical with polyphenol oxidase preparations.
 
URI
http://hdl.handle.net/1842/35298
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  • Chemistry thesis and dissertation collection

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