Studies on vitamin C

dc.contributor.author	Meiklejohn, George T.	en
dc.date.accessioned	2019-02-15T14:36:44Z
dc.date.available	2019-02-15T14:36:44Z
dc.date.issued	1940	en
dc.identifier.uri	http://hdl.handle.net/1842/35298
dc.description.abstract		en
dc.description.abstract	The oxidase activity of cucumber juice is proportional to the amount of copper in the juice after all the "free" copper and other extraneous materials have been removed by prolonged dialysis against pure water. This activity is much greater than the catalytic power of copper in the ionic state or copper in loose non-specific combination with protein. Removal of the copper present in cucumber juice after long dialysis against water by further dialysis against cyanide or dilute mineral acid inactivates the oxidase. Oxidase preparations inactivated by dialysis against cyanide are restored to activity by the addition of small excesses of copper sulphate, the restored enzyme showing greater catalytic activity than the amount of copper effecting the restoration. It is concluded that cucumber juice contains a discrete ascorbic acid oxidase, a metal protein in which the prosthetic group is copper and which is not identical with polyphenol oxidase preparations.	en
dc.publisher	The University of Edinburgh	en
dc.relation.ispartof	Annexe Thesis Digitisation Project 2019 Block 22	en
dc.relation.isreferencedby		en
dc.title	Studies on vitamin C	en
dc.type	Thesis or Dissertation	en
dc.type.qualificationlevel	Doctoral	en
dc.type.qualificationname	PhD Doctor of Philosophy	en