Show simple item record

dc.contributor.advisorSmith, Stewart
dc.contributor.advisorWalton, Anthony
dc.contributor.advisorMurray, Alan
dc.contributor.authorJamil, Nadira
dc.date.accessioned2019-08-07T12:04:32Z
dc.date.available2019-08-07T12:04:32Z
dc.date.issued2019-07-03
dc.identifier.urihttp://hdl.handle.net/1842/36001
dc.description.abstractThis research involves the design and fabrication of micro-scale electrodes and optimisation of image reconstruction techniques. It aims to explore the use of bioimpedance tomography techniques in extracting some structured information on three-dimensional (3D) cell growth for the purpose of identifying cancer development, such as, cancer cell spheroids. Electrical impedance tomography (EIT) is a non-invasive imaging technique that maps the variation in conductivity of a sample, in the form of two or three dimensions. This technique has been successfully used in many clinical applications, for example, in detection of breast cancer, acute stroke differentiation, detection of bleeding due to traumatic brain injury, and detection of bacterial infection during surgery. The capability of EIT to spatially map biological development process enables it to be used in monitoring cell growth in three-dimensional formation. The work presented in this thesis includes miniaturising the electrode designs from a millimetre-scale on a PCB to a micrometre-scale on a glass substrate, and on a flexible material. Apart from the fabrication and experimental work, sensitivity analysis was performed using COMSOL Multiphysics® modelling. The final electrode design, the flexible micro-scale electrode array (Flex-MEA), is fabricated on a flexible printed circuit board (PCB). The development of Flex-MEA technology with improved imaging reconstruction on micro-scale has produced an improved high-throughput and showed great potential as a research aid in drug discovery. The research has proven that Flex-MEA enables improved electrode arrangement compared with planar Pt electrodes making it a superior choice as a portable, non-invasive technique to image the growth of microbial cultures. Successful measurements of cell growth and proliferation propounded by this research will have a definite potential not only in the biomedical field, example, in therapeutic drug monitoring, but also in bioprocessing technology.en
dc.contributor.sponsorEngineering and Physical Sciences Research Council (EPSRC)en
dc.language.isoenen
dc.publisherThe University of Edinburghen
dc.relation.hasversionY. Yang, J. Jia, S. Smith, N. Jamil, W. Gamal & P.O. Bagnaninchi, “A Miniature Electrical Impedance Tomography Sensor and 3-D Image Reconstruction for Cell Imaging”, IEEE Sensors Journal, vol. 17, no. 2, pp. 514-523, 2017.en
dc.relation.hasversionN. Jamil, Y. Yang, M. Cela, L.P. Basanta, E.O. Blair, S. Dimartino, J. Jia & S. Smith, “Flexible microelectrode array (Flex-MEA) design for micro-bioimpedance tomography of microbial cultures”, 4th Conference on Impedance-Based Cellular Assays, Edinburgh, Scotland, June 2018.en
dc.relation.hasversionN. Jamil, Y. Yang, A. Tsiamis, J. Jiabin, & S. Smith, “Comparison of Regularisation Methods in Image Reconstruction for Micro-Bioimpedance Tomography”, IEEE Sensors, Glasgow, Scotland, October 2017.en
dc.relation.hasversionN. Jamil, Y. Yang, A. Tsiamis, J. Jiabin, & S. Smith, “Design and fabrication of different platinum microelectrodes morphologies for electrical impedance tomography in biomedical applications”, 5th International Conference on Bio-Sensing Technology, Riva del Garda, Italy, May 2017.en
dc.relation.hasversionN. Jamil, S. Smith, Y. Yang, J. Jia, P.O. Bagnaninchi & E. González-Fernández, “Design and fabrication of microelectrodes for electrical impedance tomography of cell spheroids”, IEEE EMBS Conference on Biomedical Engineering and Sciences, pp. 426- 431, Kuala Lumpur, Malaysia, December 2016.en
dc.relation.hasversionN. Jamil, Y. Yang, J. Jia, A.F. Murray, & S. Smith, “Fabrication of gold-plated electrodes on a printed circuit board (PCB) for electrical impedance tomography (EIT) measurement in fluid environment”, MicroTech 2016 - Sensors, MEMS & Advanced Packaging, Edinburgh, Scotland, March 2016.en
dc.relation.hasversionA. Buchoux, N. Jamil, A. Tsiamis & S. Smith, “Packaging of Silicon Chip by Selectively Curing a Photosensitive Acrylic Resin with a DLP Projector”, MicroTech 2016 - Sensors, MEMS & Advanced Packaging, Edinburgh, Scotland, March 2016.en
dc.relation.hasversionA. Tsiamis, Y. Li, N. Jamil, S. Smith, H.M. Reekie, J. Terry, A.J. Walton & A.F. Murray, “On-Chip Post-Processing Fabrication Techniques for Extended Gate ISFET pH Sensors”, 4th International Conference on Bio-Sensing Technology, Lisbon, Portugal, May 2015.en
dc.subjectmicro-scale electrode arraysen
dc.subjectmicro-bioimpedance tomographyen
dc.subjectmicrofabricationen
dc.subjectMCF7en
dc.subjectcell spheroidsen
dc.titleDesign, modelling and fabrication of micro-scale electrode arrays (MEAs) for micro-bioimpedance tomographyen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


Files in this item

This item appears in the following Collection(s)

Show simple item record