dc.contributor.advisor | Allshire, Robin | en |
dc.contributor.advisor | Matthews, Keith | en |
dc.contributor.author | Staneva, Desislava Plamenova | en |
dc.date.accessioned | 2020-05-01T10:37:26Z | |
dc.date.available | 2020-05-01T10:37:26Z | |
dc.date.issued | 2020-07-04 | |
dc.identifier.uri | https://hdl.handle.net/1842/37025 | |
dc.identifier.uri | http://dx.doi.org/10.7488/era/326 | |
dc.description.abstract | In eukaryotic nuclei, DNA is packaged into chromatin by association with histone proteins.
Specific histone post-translational modifications result in the formation of transcriptionally
active and silent chromatin domains. The aim of this project was to characterise chromatin
organisation and assess the role of transcription regulation in Trypanosoma brucei, the
causative agent of sleeping sickness in Africa. The approach taken was endogenous
fluorescent tagging of putative readers, writers and erasers of histone modifications in T.
brucei and subsequent use of the tags to localise the candidate proteins in the cell and to
identify their genomic associations and protein interaction networks. Chromatin
immunoprecipitation followed by sequencing showed that many of the nuclear proteins
associate with RNAPII transcription start regions (TSRs). Whereas most proteins were
enriched broadly over those regions, Chromo1 and SET27 displayed sharp overlapping peak
profiles. Moreover, co-immunoprecipitation and mass spectrometry revealed that Chromo1
and SET27 are likely part of the same complex together with four uncharacterised proteins
and JBP2, an enzyme involved in the synthesis of the DNA modification base J. Overall, this
work provides the basis for investigating the role of chromatin factors in trypanosome
transcription regulation. | en |
dc.contributor.sponsor | Wellcome Trust | en |
dc.language.iso | en | |
dc.publisher | The University of Edinburgh | en |
dc.subject | gene regulation | en |
dc.subject | Trypanosoma brucei | en |
dc.subject | endogenous fluorescent tagging | en |
dc.subject | TSRs | en |
dc.subject | SET27 | en |
dc.title | Chromatin organisation and transcription regulation in Trypanosoma brucei | en |
dc.type | Thesis or Dissertation | en |
dc.type.qualificationlevel | Doctoral | en |
dc.type.qualificationname | PhD Doctor of Philosophy | en |
dc.rights.embargodate | 2021-07-04 | |
dcterms.accessRights | Restricted Access | en |