Edinburgh Research Archive logo

Edinburgh Research Archive

University of Edinburgh homecrest
View Item 
  •   ERA Home
  • Chemistry, School of
  • Chemistry thesis and dissertation collection
  • View Item
  •   ERA Home
  • Chemistry, School of
  • Chemistry thesis and dissertation collection
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Fluorescent probes for microorganisms

View/Open
BaibekA_2022.pdf (10.71Mb)
Date
28/06/2022
Item status
Restricted Access
Embargo end date
28/06/2023
Author
Baibek, Assel
Metadata
Show full item record
Abstract
One of the main problems with infectious diseases is their late and unspecific diagnosis. To overcome this problem, optical molecular imaging can be applied using target selective fluorescent probes. The focus of this work has been the development of a series of fluorescent probes for microorganism detection that can be applied in a number of clinical scenarios allowing rapid diagnosis. In the first part of this work, a set of fluorogenic probes was synthesised based on an antimicrobial peptide. These probes show the labelling of bacteria and fungi and do not require any washing steps. The use of D-amino acids provides high proteolytic stability. Another set of probes was developed based on the antifungal drug amphotericin B. They were assessed on the labelling of a range of fungal species, including clinically isolated strains and biofilms. Selective labelling of fungi in comparison with bacteria has allowed the application of red-emitting fungal probe together with reported bacteria-specific probes for multiplexed imaging of a fungi-bacteria mixture. A library of probes for selective labelling of mycobacteria was synthesised using a range of fluorophores (covering the spectral window of 400 to 650 nm) and trehalose molecule. It has been shown that mycobacteria can uptake endogenous and exogenous trehalose and incorporate it into mycomembrane. Two probes with spectral overlap were utilised as FRET pair for imaging of mycobacteria. All of the developed probes showed efficient labelling of microorganisms and can be used as a foundation for the development of future rapid diagnostic techniques.
URI
https://hdl.handle.net/1842/39221

http://dx.doi.org/10.7488/era/2472
Collections
  • Chemistry thesis and dissertation collection

Library & University Collections HomeUniversity of Edinburgh Information Services Home
Privacy & Cookies | Takedown Policy | Accessibility | Contact
Privacy & Cookies
Takedown Policy
Accessibility
Contact
feed RSS Feeds

RSS Feed not available for this page

 

 

All of ERACommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsPublication TypeSponsorSupervisorsThis CollectionBy Issue DateAuthorsTitlesSubjectsPublication TypeSponsorSupervisors
LoginRegister

Library & University Collections HomeUniversity of Edinburgh Information Services Home
Privacy & Cookies | Takedown Policy | Accessibility | Contact
Privacy & Cookies
Takedown Policy
Accessibility
Contact
feed RSS Feeds

RSS Feed not available for this page