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dc.contributor.advisorMarston, Adele
dc.contributor.authorVincenten, Nadine
dc.date.accessioned2023-05-08T12:50:51Z
dc.date.available2023-05-08T12:50:51Z
dc.date.issued2015
dc.identifier.urihttps://hdl.handle.net/1842/40556
dc.identifier.urihttp://dx.doi.org/10.7488/era/3321
dc.description.abstractThe segregation of homologous chromosomes during meiosis I is a key step in the formation of gametes. Proper segregation of homologs requires correct bipolar spindle attachment. However, how a cell monitors and achieves this remains unclear. This work explores the role of the conserved Ctf19 kinetochore complex in ensuring the accurate segregation of homologous chromosomes during meiosis I in Saccharomyces cerevisiae. The Ctf19 complex was found to contribute to faithful chromosome segregation during meiosis in at least three ways. First, it was found that the Ctf19 complex is required for the localisation of both cohesin as well as the synaptonemal complex protein, Zip1, to the centromere. Consistent with the reduced levels of cohesin at the centromere, the disjunction of sister chromatids in meiosis II is impaired in Ctf19 complex mutants. Second, it was shown that a functional Ctf19 complex is important to repress crossover formation in a pericentromeric interval, but not in a chromosome arm interval. Third, preliminary data indicates that Ctf19 complex members are required for robust kinetochoremicrotubule attachments. However, both the spindle assembly checkpoint and the Aurora B-dependent error correction machinery appear to be functional in Ctf19 complex mutants. Overall, these findings demonstrate that the Ctf19 complex plays critical roles during both meiotic prophase I and metaphase I that impinge on the accurate segregation of homologous chromosomes during meiosis I.en
dc.contributor.sponsorWellcome Trusten
dc.language.isoenen
dc.publisherThe University of Edinburghen
dc.subjectCTF19en
dc.subjectCHROMOSOME SEGREGATIONen
dc.subjectS. CEREVISIAEen
dc.subjectS. CEREVISIAE MEIOSISen
dc.subjecthomologous chromosomesen
dc.subjectmeiosis Ien
dc.subjecthomologsen
dc.subjectbipolar spindle attachmenten
dc.subjectCtf19 kinetochore complexen
dc.titleCTF19 complex ensures accurate chromosome segregation by directing multiple key processes during S. cerevisiae meiosisen
dc.title.alternativeThe CTF19 complex ensures accurate chromosome segregation by directing multiple key processes during S. cerevisiae meiosisen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen
dcterms.accessRightsRestricted Accessen


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