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dc.contributor.authorNiwa, Hitoshi
dc.contributor.authorMasui, Shinji
dc.contributor.authorChambers, Ian
dc.contributor.authorSmith, Austin G
dc.contributor.authorMiyazaki, Jun-ichi
dc.coverage.spatial11en
dc.date.accessioned2005-01-06T10:12:20Z
dc.date.available2005-01-06T10:12:20Z
dc.date.issued2001
dc.identifier.citationMOLECULAR AND CELLULAR BIOLOGY, Mar. 2002, p. 1526–1536en
dc.identifier.uriDOI: 10.1128/MCB.22.5.1526–1536.2002
dc.identifier.urihttp://hdl.handle.net/1842/645
dc.description.abstractTranscription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The POU factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal of pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, and A. G. Smith, Nat. Genet. 24:372–376, 2000). Here we have developed a complementation assay based on the ability of Oct-3/4 transgenes to rescue self-renewal in conditionally null ES cells and used this to define which domains of Oct-3/4 are required to sustain the undifferentiated stem cell phenotype. Surprisingly, we found that molecules lacking either the N-terminal or C-terminal transactivation domain, though not both, can effectively replace full-length Oct-3/4. Furthermore, a fusion of the heterologous transactivation domain of Oct-2 to the Oct-3/4 POU domain can also sustain self-renewal. Thus, the unique function of Oct-3/4 in ES cell propagation resides in combination of the specific POU domain with a generic proline-rich transactivation domain. Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation.en
dc.format.extent393150 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherCopyright © 2002, American Society for Microbiology. All Rights Reserved.en
dc.subjectPhenotypic Complementationen
dc.subjectEmbryonicen
dc.subjectStem Cellsen
dc.subjectgeneen
dc.titlePhenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cellsen
dc.typeArticleen


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