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dc.contributor.advisorArchibald, Alanen
dc.contributor.advisorAshworth, Cherylen
dc.contributor.advisorHaley, Christopheren
dc.contributor.authorHernández Velasco, Silvia Claraen
dc.date.accessioned2012-10-02T15:04:44Z
dc.date.available2012-10-02T15:04:44Z
dc.date.issued2012-06-30
dc.identifier.urihttp://hdl.handle.net/1842/6467
dc.description.abstractFemale reproductive performance is a critical component of sustainable pig production systems. There is abundant evidence of genetic variation in these traits among pig breeds. The aims of this study were to identify quantitative trait loci (QTL) affecting reproductive traits and to identify and characterise positional candidate gene(s) underlying the QTL. A Large White - Meishan F2 population was scanned for QTL with effects on reproductive traits. This analysis revealed 13 putative QTLs on seven different chromosomes with effects on five different traits: ovulation rate (OR), teat number (TN), prenatal survival (PS), total born alive (TBA) and litter size (LS). QTL for PS and LS on chromosome 8 were fine mapped and Secreted Phosphoprotein 1 (SPP1) confirmed as a candidate gene. A genome-wide association study was performed on a diverse population of different breeds and crosses lines, for reproductive traits including LS, TBA, number of stillborn piglets, and number of mummified piglets. Fourteen SNPs were found significantly associated with reproductive traits. The functional study of SPP1 examined the hypothesis that differences in foetal growth may be associated with the effectiveness of conceptus attachment, as measured by SPP1 expression. Patterns of SPP1 mRNA and protein expression in placental and uterine tissues supplying the smallest and a normal-sized foetus from the same uterus were examined in Large White-Landrace (LW-LR), Large White (LW) and Meishan (MS) females 40 and 45 of pregnancy. The smallest LW-LR foetuses tended to have a higher level of SPP1 mRNA in endometrium tissue compared to the normal-sized foetuses. However, placenta expression was higher in the normal-sized foetuses compared to the smallest ones. SPP1 protein levels in normal sized foetuses were significantly higher than in the smallest litter mates for all the tissues. Significantly higher levels of SPP1 mRNA and protein were found in MS compared to LW. In both breeds, significant differences between sizes were found in some tissues, with similar expression patterns in respect to size, for both mRNA and protein in endometrial tissues when compared to contemporary LW. In placenta, the direction of the expression differed between breeds, with a higher expression of mRNA and protein in the normal-sized MS foetuses and in the smallest sized LW foetuses. The comparison of SPP1 expression between different foetal sizes and different breeds revealed associations between breed, foetal size, and SPP1 protein, factors implicated in PS and LS. These results together with the genetic evidence indicate that the potential role of SPP1 in placental and foetal development merits further investigation.en
dc.contributor.sponsorBiotechnology and Biological Sciences Research Council (BBSRC)en
dc.language.isoen
dc.publisherThe University of Edinburghen
dc.relation.hasversionHernandez, S.C., Hogg, C.O., Billon, Y., Bidanel, J.P., Haley, C.S., Archibald, A.L. and Ashworth, C.J. Localisation and expression of Secreted Phosphoprotein 1 in uterine and placental tissues from Large White and Meishan pigs. Proceedings for the Annual Conference of the Society for Reproduction and Fertility, Brighton 2011. Abstract O24en
dc.relation.hasversionHernandez, S.C., Finlayson, H.A., Hogg, C.O., Ashworth, C.J., Haley, C.S. and Archibald, A.L. Genetics of litter size and embryo survival in pigs. Proceedings for the 32nd Conference for the International Society for Animal Genetics, Edinburgh 2010. Abstract P4028 pp 98en
dc.relation.hasversionHernandez, S.C., Hogg, C.O., Khazanchi, N., Haley, C.S., Archibald, A.L. and Ashworth, C.J. Localization and expression of SPP1 in porcine uterine and placental tissues. Proceedings for the Annual Conference of the Society for Reproduction and Fertility, Nottingham 2010. Abstract P78 p83en
dc.relation.hasversionHernandez, S.C., Finlayson, H.A., Ashworth, C.J., Haley, C.S. and Archibald, A.L. Mapping Quantitative Trait Loci for reproduction in pigs Society of Reproduction and Fertility Supplement, 2009; Vol. 66. Control of Pig Reproduction VIII, Nottingham University Press. pp 117-118en
dc.subjectpigsen
dc.subjectlitter sizeen
dc.subjectprenatal survivalen
dc.subjectovulation rateen
dc.subjectsingle nucleotide polymorphismsen
dc.subjectSNPsen
dc.subjectteat numberen
dc.subjectSecreted Phosphoprotein 1en
dc.subjectSPP1en
dc.subjectQTLen
dc.subjectquantitative trait locien
dc.subjectplacentaen
dc.subjectendometriumen
dc.titleGenetics of litter size and prenatal survival in pigsen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


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