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dc.contributor.authorLeng, J
dc.contributor.authorEgelhaaf, Stefan U
dc.contributor.authorCates, ME
dc.coverage.spatial23en
dc.date.accessioned2005-02-01T11:04:24Z
dc.date.available2005-02-01T11:04:24Z
dc.date.issued2003-09
dc.identifier.citationBiophysical Journal (2003), Volume 85(3), 1624–1646en
dc.identifier.urihttp://hdl.handle.net/1842/671
dc.description.abstractImportant routes to lipid vesicles (liposomes) are detergent removal techniques, such as dialysis or dilution. Although they are widely applied, there has been only limited understanding about the structural evolution during the formation of vesicles and the parameters that determine their properties. We use time-resolved static and dynamic light scattering to study vesicle formation in aqueous lecithin-bile salt mixtures. The kinetic rates and vesicle sizes are found to strongly depend on total amphiphile concentration and, even more pronounced, on ionic strength. The observed trends contradict equilibrium calculations, but are in agreement with a kinetic model that we present. This model identifies the key kinetic steps during vesicle formation: rapid formation of disklike intermediate micelles, growth of these metastable micelles, and their closure to form vesicles once line tension dominates bending energy. A comparison of the rates of growth and closure provides a kinetic criterion for the critical size at which disks close and thus for the vesicle size. The model suggests that liposomes are nonequilibrium, kinetically trapped structures of very long lifetime. Their properties are hence controlled by kinetics rather than thermodynamics.en
dc.format.extent375628 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherThe Biophysical Societyen
dc.subjectKineticsen
dc.subjectmicelleen
dc.subjectBiophysical Chemistryen
dc.subjectvesicleen
dc.titleKinetics of the Micelle-to-Vesicle Transition: Aqueous Lecithin-Bile Salt Mixturesen
dc.typeArticleen


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