Show simple item record

dc.contributor.authorAlexeev, Dmitriy
dc.contributor.authorZhu, Haizhong
dc.contributor.authorGuo, Maolin
dc.contributor.authorZhong, Weiqing
dc.contributor.authorHunter, Dominic JB
dc.contributor.authorYang, Weiping
dc.contributor.authorCampopiano, Dominic J
dc.contributor.authorSadler, Peter J
dc.coverage.spatial7en
dc.date.accessioned2005-02-01T16:14:46Z
dc.date.available2005-02-01T16:14:46Z
dc.date.issued2003
dc.identifier.citationA novel protein-mineral interface. Alexeev, D.; Zhu, H.; Guo, M.; Zhong, W.; Hunter, D. J. B.; Yang, W.; Campopiano, D. J.; Sadler, P. J. Nature Structural Biology (2003), 10(4), 297-302.en
dc.identifier.uriDOI: 10.1038/nsb903
dc.identifier.urihttp://hdl.handle.net/1842/677
dc.description.abstractTransferrins transport Fe3+ and other metal ions in mononuclear-binding sites. We present the first evidence that a member of the transferrin superfamily is able to recognize multi-nuclear oxo-metal clusters, small mineral fragments that are the most abundant forms of many metals in the environment. We show that the ferric ion-binding protein from Neisseria gonorrhoeae (nFbp)readily binds clusters of Fe3+, Ti4+, Zr4+ of Hf4+ in solution. The 1.7A resolution crystal structure of Hf-nFbp reveals three distinct types of clusters in an open, positively charged cleft between two hinged protein domains. A di-tyrosol cluster nucleation motif (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear cluster, which in turn can be capped with phosphate. This first high-reesolution structure of a protein-mineral interface suggests a novel metal-uptake mechanism and provides a model for protein-mediated mineralization/dissimilation, which plays a critical role in geochemical processes.en
dc.format.extent925617 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherNature Publishing Groupen
dc.subjectMineralen
dc.subjectProteinen
dc.subjecttransferrinen
dc.titleA novel protein-mineral interface.en
dc.typeArticleen


Files in this item

This item appears in the following Collection(s)

Show simple item record