dc.contributor.author | McNeilly, Jane D | |
dc.contributor.author | Heal, Mathew R | |
dc.contributor.author | Beverland, Iain J | |
dc.contributor.author | Howe, Alan | |
dc.contributor.author | Gibson, Mark D | |
dc.contributor.author | Hibbs, Leon | |
dc.contributor.author | MacNee, William | |
dc.contributor.author | Donaldson, Ken | |
dc.coverage.spatial | 25 | en |
dc.date.accessioned | 2005-10-05T14:45:19Z | |
dc.date.available | 2005-10-05T14:45:19Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | McNeilly, J. D., Heal, M. R., Beverland, I. J., Howe, A., Gibson, M. D., Hibbs, L. R., MacNee, W. and Donaldson, K. (2004) Soluble transition metals cause the pro-inflammatory effects of welding fumes in vitro, Toxicology and Applied Pharmacology 196, 95-107 | en |
dc.identifier.uri | DOI: 10.1016/j.taap.2003.11.021 | |
dc.identifier.uri | http://hdl.handle.net/1842/841 | |
dc.description.abstract | Epidemiological studies have consistently reported a higher incidence of respiratory
illnesses such as bronchitis, metal fume fever (MFF), and chronic pneumonitis among
welders exposed to high concentrations of metal-enriched welding fumes. Here, we
studied the molecular toxicology of three different metal-rich welding fumes:
NIMROD 182, NIMROD c276, and COBSTEL 6. Fume toxicity in vitro was
determined by exposing human type II alveolar epithelial cell line (A549) to whole
welding fume, a soluble extract of fume or the "washed" particulate. All whole fumes
were significantly toxic to A549 cells at doses >63 μg ml−1 (TD 50; 42, 25, and 12 μg
ml−1, respectively). NIMROD c276 and COBSTEL 6 fumes increased levels of IL-8
mRNA and protein at 6 h and protein at 24 h, as did the soluble fraction alone,
whereas metal chelation of the soluble fraction using chelex beads attenuated the
effect. The soluble fraction of all three fumes caused a rapid depletion in intracellular
glutathione following 2-h exposure with a rebound increase by 24 h. In addition, both
nickel based fumes, NIMROD 182 and NIMROD c276, induced significant reactive
oxygen species (ROS) production in A549 cells after 2 h as determined by DCFH
fluorescence. ICP analysis confirmed that transition metal concentrations were similar
in the whole and soluble fractions of each fume (dominated by Cr), but significantly
less in both the washed particles and chelated fractions. These results support the
hypothesis that the enhanced pro-inflammatory responses of welding fume
particulates are mediated by soluble transition metal components via an oxidative
stress mechanism. | en |
dc.format.extent | 768486 bytes | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.publisher | Elsevier | en |
dc.subject | Welding fumes | en |
dc.subject | Transition metals | en |
dc.subject | Inflammation | en |
dc.subject | Oxidative stress | en |
dc.title | Soluble transition metals cause the pro-inflammatory effects of welding fumes in vitro | en |
dc.type | Article | en |