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dc.contributor.authorMcNeilly, Jane D
dc.contributor.authorHeal, Mathew R
dc.contributor.authorBeverland, Iain J
dc.contributor.authorHowe, Alan
dc.contributor.authorGibson, Mark D
dc.contributor.authorHibbs, Leon
dc.contributor.authorMacNee, William
dc.contributor.authorDonaldson, Ken
dc.coverage.spatial25en
dc.date.accessioned2005-10-05T14:45:19Z
dc.date.available2005-10-05T14:45:19Z
dc.date.issued2004
dc.identifier.citationMcNeilly, J. D., Heal, M. R., Beverland, I. J., Howe, A., Gibson, M. D., Hibbs, L. R., MacNee, W. and Donaldson, K. (2004) Soluble transition metals cause the pro-inflammatory effects of welding fumes in vitro, Toxicology and Applied Pharmacology 196, 95-107en
dc.identifier.uriDOI: 10.1016/j.taap.2003.11.021
dc.identifier.urihttp://hdl.handle.net/1842/841
dc.description.abstractEpidemiological studies have consistently reported a higher incidence of respiratory illnesses such as bronchitis, metal fume fever (MFF), and chronic pneumonitis among welders exposed to high concentrations of metal-enriched welding fumes. Here, we studied the molecular toxicology of three different metal-rich welding fumes: NIMROD 182, NIMROD c276, and COBSTEL 6. Fume toxicity in vitro was determined by exposing human type II alveolar epithelial cell line (A549) to whole welding fume, a soluble extract of fume or the "washed" particulate. All whole fumes were significantly toxic to A549 cells at doses >63 μg ml−1 (TD 50; 42, 25, and 12 μg ml−1, respectively). NIMROD c276 and COBSTEL 6 fumes increased levels of IL-8 mRNA and protein at 6 h and protein at 24 h, as did the soluble fraction alone, whereas metal chelation of the soluble fraction using chelex beads attenuated the effect. The soluble fraction of all three fumes caused a rapid depletion in intracellular glutathione following 2-h exposure with a rebound increase by 24 h. In addition, both nickel based fumes, NIMROD 182 and NIMROD c276, induced significant reactive oxygen species (ROS) production in A549 cells after 2 h as determined by DCFH fluorescence. ICP analysis confirmed that transition metal concentrations were similar in the whole and soluble fractions of each fume (dominated by Cr), but significantly less in both the washed particles and chelated fractions. These results support the hypothesis that the enhanced pro-inflammatory responses of welding fume particulates are mediated by soluble transition metal components via an oxidative stress mechanism.en
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dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherElsevieren
dc.subjectWelding fumesen
dc.subjectTransition metalsen
dc.subjectInflammationen
dc.subjectOxidative stressen
dc.titleSoluble transition metals cause the pro-inflammatory effects of welding fumes in vitroen
dc.typeArticleen


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