Show simple item record

dc.contributor.advisorCampbell, Colinen
dc.contributor.advisorMowat, Christopheren
dc.contributor.authorAuchinvole, Craig Alexander Ren
dc.date.accessioned2014-11-21T15:48:02Z
dc.date.available2014-11-21T15:48:02Z
dc.date.issued2012-06-22
dc.identifier.urihttp://hdl.handle.net/1842/9706
dc.description.abstractRedox regulation and homeostasis are critically important in the regulation of cell function; however, there are significant challenges in quantitatively measuring and monitoring intracellular redox potentials. The work in this thesis details a novel approach to intracellular redox monitoring. The approach is based on the use of nanosensors, which comprise molecules capable of sensing the local redox potential, assembled on gold nanoshells. Since the Raman spectra of the sensor molecules change depending on their oxidation state, and since the nanoshells allow a large enhancement of the Raman scattering, intracellular potential can be calculated by simple optical measurements. A full description of the design, fabrication and characterisation (spectroscopic and electrochemical) of the nanosensors is provided within. The ability to deliver nanosensors into cells in a controllable fashion was confirmed using electron microscopy. Results from a range of assays are also presented which reveal that introduction of nanosensors does not result in any cytotoxicity. Sensor utility in monitoring redox potentials as cells responded to physiological and superphysiological oxidative and reductive stimuli was investigated. Importantly, the capability of the nanosensors in monitoring intracellular potentials in a reversible, non-invasive manner, and over a previously unattainable potential range, is demonstrated.en
dc.contributor.sponsorEngineering and Physical Sciences Research Council (EPSRC)en
dc.language.isoen
dc.publisherThe University of Edinburghen
dc.subjectredox regulationen
dc.subjecthomeostasisen
dc.subjectintracellular redox monitoringen
dc.subjectnanosensorsen
dc.subjectRaman scatteringen
dc.titleSERS nanosensors for intracellular redox potential measurementsen
dc.typeThesis or Dissertationen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD Doctor of Philosophyen


Files in this item

This item appears in the following Collection(s)

Show simple item record