A method of measuring adsorption without
washing the tissue has been devised and
Diffusion through chopped lung tissue has
been studied from a theoretical and
experimental point of view. Predicted
curves for the y-globulin content of the
extracellular space have been calculated
and applied to experimental results. The
rate of uptake was found to be at least
partly diffusion controlled. Adsorption
equilibrium and rate constants have been
rigorously defined. No evidence was found
for a fast initial phase of sensitization
Calculations have been performed concerning
the consequences of slow steady y-globulin
uptake after long periods of incubation.
The amounts of rabbit y-globulin adsorbed
onto lung tissue were found to be of the
same order of magnitude, or somewhat lower,
than those previously reported.
No evidence against a linear adsorption
isotherm was found in any experiments.
Neither adsorption nor sensitization were
altered by reducing the calcium concentration during passive sensitization.
Reduction of the ionic strength of the
medium caused a large increase in the amount
of Y-globulin adsorbed but did not increase
Two guinea pig antibodies have been
separated by preparative electrophoresis
and ion exchange chromatography.
The antigenic relationship and purity of
γ₁-and γ₂-globulins have been studied. No
contamination was detectable in γ₂_globulin,
but y₁-globulin contained fast γ₂-globulin
and sometimes ß-globulins as well.
Ovalbumin (crystallized 5 times) has been
shown to contain at least four proteins.
It has been shown that γ₁-globalin anti-bodies sensitize lung tissues in very low
concentrations, but no evidence was found
that the very low sensitizing power of
γ₂-globulin antibodies was not due to
Quantitative passive cutaneous anaphylaxis
experiments performed in parallel with the
tests on lung tissue showed that whenever the γ₂-globulin fraction contained antibody it appeared more potent relative to
γ₂-globulin than when tested on lung tissue
although the γ₁-globulin was always considerably more potent in both tests.
The skin sensitization produced by the
γ₂--globulin fraction disappeared faster
than that produced by the γ₁-globulin
It was not possible to detect enough
γ₁-globulin contaminant in the γ₂-globulin
fraction to account for the skin
sensitizing ability of the latter.
It was concluded that γ₂-globulin antibody
must have some skin sensitizing ability of
its own, but that it is considerably less
potent than the γ₁-globulin antibody.
No difference was detectable between the
extents of adsorption of γ₁-and γ₂-globulins onto lung tissue.
An equation has been derived describing
the loss of thiosulphate by radiative
oxidation in iodine-131 solutions.
An analysis has been presented of interpolation and other errors in a rapid method
for assaying large numbers of histamine
solutions using visual linear interpolation
between two standards.