Marston, Adele

Earnshaw, Bill

Kocakaplan, Dilara

Darwin Trust of Edinburgh

2025-09-09T15:18:30Z

2025-09-09T15:18:30Z

2025-09-09

Cohesin loading is crucial for sister chromatid cohesion, genome organization and transcription. In budding yeast, the cohesin loader complex is composed of Scc2NIPBL and Scc4MAU2. Previous work on mitotic cells identified a conserved patch on the Scc4 that was specifically important for cohesin loading at centromeres, but not on chromosome arms. However, in meiosis, Scc4 patch was shown to be important not only for centromeric loading, but also for genome-wide loading. This suggests that the Scc4-patch targets cohesin loading to additional sites in meiosis. Furthermore, both conversion and de novo cohesion establishment pathways are required in meiosis, unlike mitosis. I hypothesize that Scc4-patch mediated meiosis-specific mode of cohesin loading may facilitate the de novo cohesion establishment pathway at replication forks. In this thesis, I aim to elucidate the molecular mechanism of Scc4-patch dependent cohesion establishment pathway in meiosis.

https://hdl.handle.net/1842/43951

http://dx.doi.org/10.7488/era/6482

en

The University of Edinburgh

2026-09-09

Cohesin loading

Meiosis

Scc4

Sister chromatid cohesion

De novo cohesion

Mechanisms of cohesin loading in meiosis

Thesis or Dissertation

Doctoral

PhD Doctor of Philosophy

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