Chromaffin granule membrane

Abstract

1. Chromaffin granule membranes have been prepared from bovine adrenal medullae. Sodium dodecyl sulphate polyacrylamide gel elctrophoresis of the membranes, in the presence of dithiothreitol, revealed 66 bands ranging in molecular weight c 150,000 to 10,000 daltons. The maajority of the bands were minor staining components. 2. The granule membranes also contain several glycoproteins (ten) which could be stained in gels of the membranes by a variety of methods (the periodic acid - Schiff, dansyl hydrazine and fluorescein isothiocyanate conjugated concanavilin A and wheat germ agglutinin stains and tritration with [3H]-borohydride following periodate or galactose oxidase treatment of the membranes).