Synthetic biology approach to monitoring transient interactions between cancer and immune cells
dc.contributor.advisor
Cachat, Elise
dc.contributor.advisor
Qian, Binzhi
dc.contributor.author
Semeniuk, Sofija
dc.date.accessioned
2023-03-28T16:28:38Z
dc.date.available
2023-03-28T16:28:38Z
dc.date.issued
2023-03-28
dc.description.abstract
Immune cells play an important role in tumour growth and progression,
as well as establishment at metastatic sites. Although inherently, immune system is
designed to locate, target and eliminate malignant cells, evolutionary processes
within a host allow tumourigenic cells to develop mechanisms and pathways to
avoid immune recognition. There is a substantial amount of knowledge on how
particular immune cell subtypes contribute to cancer growth and progression.
Specifically, macrophages play an important role in mitigating immune response
and induction of anti-inflammatory response. Due to this reason, macrophages can
become potential new therapeutic targets. However, the knowledge of underlying
mechanisms is limited due to the absence of robust tools for studying transient cell-cell interactions between cancer cells and macrophages at tumour
microenvironment. Recent advances in synthetic biology have introduced a vast
array of tools, particularly synthetic receptors, which have reported a broad range
of applications in biosensing. One of such tools is synNotch receptor, which is
derived from the core of the Notch receptor and is activated by cell-cell contact.
Both extracellular and intracellular domains of synNotch can be substituted with
custom sensing and signal transduction domains to carry out custom input/output
circuits. In this thesis, the aim is to repurpose synNotch to detect interactions
between cancer cells and macrophages in aims to develop a robust tool to aid in
studying the mechanisms of metastasis development and bring insights into
potential therapeutic targets.
en
dc.identifier.uri
https://hdl.handle.net/1842/40451
dc.identifier.uri
http://dx.doi.org/10.7488/era/3219
dc.language.iso
en
en
dc.publisher
The University of Edinburgh
en
dc.subject
mammalian synthetic biology
en
dc.subject
synthetic biology
en
dc.subject
engineering biology
en
dc.subject
synthetic receptors
en
dc.subject
cell lines
en
dc.subject
synNotch
en
dc.subject
macrophages
en
dc.subject
TME
en
dc.subject
CD206
en
dc.title
Synthetic biology approach to monitoring transient interactions between cancer and immune cells
en
dc.title.alternative
A synthetic biology approach to monitoring transient interactions between cancer and immune cells
en
dc.type
Thesis or Dissertation
en
dc.type.qualificationlevel
Doctoral
en
dc.type.qualificationname
PhD Doctor of Philosophy
en
Files
Original bundle
1 - 1 of 1
- Name:
- Semeniuk2023.pdf
- Size:
- 11.82 MB
- Format:
- Adobe Portable Document Format
- Description:
This item appears in the following Collection(s)