Preparation of an antigen of Babesia rodhaini for use in the capillary tube agglutination test
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Philip, Richard Graham
Abstract
A method for the preparation of an antigen of Babesia rodhaini for use in the capillary tube agglutination test is described.
Pilot trials on the production of an antigen by ultrasonic disintegration of B. rodhaini infected erythrocytes showed the tendency for haemoglobin from lysed rat blood to crystallise. The cause of crystallisation and means by which it could be prevented were examined.
The effects upon the antigenic activity and specificity of different periods of exposure to ultrasonic radiation and of different pH values of the diluent are described. An assessment is made of the reproducibility of the test and the method of preparation of the antigen.
Antigens were tested against sera from rats which had recovered from experimental infection with Babesia rodhaini and found to give consistent results.
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