McCartney, Elinor

2019-02-15T14:35:42Z

2019-02-15T14:35:42Z

1983

The primary aim of the work reported in this thesis was to transmit intestinal adenomatosis to experimental piglets, thus enabling a study of the role of Campylobacter sputorum subspecies mucosalis (mucosalis) in the pathogenesis of the disease. Field cases of porcine intestinal adenomatosis (PIA) were studied, initially to provide experience for the author in the techniques used, and later to obtain infective mucosa and strains of mucosalis with which to orally dose experimental piglets. Naturally-farrowed, cross-suckled piglets were exposed to adenomatous mucosa and cultures of mucosalis. Mucosalis was not isolated from the enteric mucosa at necropsy and no lesions of adenomatosis were found, hence alternative approaches to the problem were tried. Naturally-farrowed, colostrum-deprived piglets were exposed to adenomatous mucosa, but did not survive for long enough to assess whether adenomatous change would have occurred. The absence of maternallyacquired immunity did not appear to enhance greatly the establishment of mucosalis in the gut. Gnotobiotic piglets were exposed to cultures of mucosalis alone or in combination with rotavirus. Mucosalis established and persisted in the lumen of the gut in both groups but lesions of adenomatosis did not develop. Weaned pigs, exposed to either dual mucosalis and rotavirus, or dual mucosalis and enterotoxigenic Esch erichia coli infections, did not develop adenomatosis and mucosalis was not isolated from the gut at necropsy. Finally a series of experiments was carried out, on both milk-fed and creep-fed piglets, to investigate whether Cryptosporidia, protozoan parasites of the surface of epithelial cells, could promote parasitism of pig enterocytes by mucosalis. There was no evidence that Cryptosporidia enhanced the establishment of mucosalis in the gut, and adenomatous change was not observed.

http://hdl.handle.net/1842/35194

The University of Edinburgh

Annexe Thesis Digitisation Project 2019 Block 22

Already catalogued

Studies on Campylobacter Sputorum subspecies Mucosalis infection in pigs

Thesis or Dissertation

Doctoral

PhD Doctor of Philosophy