Teale, Alan John

2018-05-14T10:16:39Z

2018-05-14T10:16:39Z

1983

Following an introduction and review of literature pertaining to relevant aspects of major histocompatibility systems and a review of literature on the bovine system specifically, an introduction to East Coast fever (ECF) and review of literature on ECF immunology and lymphoblastoid cell line (LCL) vaccination is given.

The development and application of a technique for selection of bovine lymphocyte antigen (BoLA) ~ defined subpopulations within established LCLs transformed by the causative organism of ECF (Theileria parva) is then described. The results are discussed in the context of T.parva-preferred target cells in in vitro transformation systems, and the possible application of the technique in producing LCLs lacking BoLA expression is considered. No evidence of modulation of BoLA phenotype (workshop specificities) as a consequence of Theileria~induced transformation was found.

Two large scale LCL challenge experiments in cattle are reported, in which degrees of compatibility between LCLs and recipient cattle were quantified and the effect of this on the responses to LCL inoculation and subsequent stabilate challenge, assessed. Evidence is presented for the BoLA system being an important factor in determining the response to LCL inoculation and the generation of immunity to a second challenge with the homologous parasite, when cattle are inoculated with low numbers of cells initially.

Also presented are the results of a series of experiments carried out to investigate the specificity of bovine alloreactive cytotoxic cells generated in vitro. The results suggest that BoLA workshop specificities, as defined by alloantisera, are restrictive in this system. The results also suggest that effects due to expression of the products of a BoLA locus other than that coding for the workshop specificities were being detected in some experiments.

The significance of these results for future studies of the bovine immune system in general, and for studies of the immune response in ECF in particular, is discussed.

http://hdl.handle.net/1842/30021

The University of Edinburgh

Annexe Thesis Digitisation Project 2018 Block 18

Already catalogued

The major histocompatibility complex of cattle with particular reference to some aspects of East Coast fever

Thesis or Dissertation

Doctoral

PhD Doctor of Philosophy