Taylor, Philip Leslie

2019-02-15T14:24:24Z

2019-02-15T14:24:24Z

1979

The lipid fraction of human semen has been separated by LH 20 Sephadex chromatography and TLC and the resulting fractions analysed by GCMS. Evidence is presented for the existence in semen of several prostaglandins, including 19 -OH PG F₁α, 19-OH PG F₂α, 8-iso 19-OH PG El, 8 -iso 19-OH PG E₂, 8 -iso PG F10, 8 -iso PG F20 , 8 -iso PG E₁ and 8-iso PG E₂. None of these compounds have previously been described in semen, and the first five have not been described from any source. Evidence is also presented for the existence of a group of prostaglandins isomeric with the dinor prostaglandins El₁, E₂, F₁α and F₂α. A method for the preparation of crystalline 19-OH PG E₁ from macaque semen is described. Evidence is presented for the existence of a pair of dihydroxy E prostaglandins, tentatively identified as 18,19 dihydroxy PG E₁ and an isomeric compound in the semen of the stump-tailed macaque. The effects of 19-OH PG E₁ on non-pregnant and pregnant human isolated myometrial strips have been studied. 19-OH PG E₁, like PG E₁, was found to relax non-pregnant strips, the equipotent molar ratio being E₁:19-OH E₁ = 1:4. 19-OH PG E₁ was also found to relax pregnant strips, unlike PG E₁, and a significant effect was demonstrated at 50 ng /ml. Examination of homogenates of the seminal vesicle of the stump tailed macaque by GCMS showed them to contain large amounts of 19-OH E prostaglandins, and these levels increased on incubation, an effect which was inhibited by indomethacin. Incubation of homogenates with radioactively-labelled eicosa-8,11,14-trienoic acid, arachidonic acid, PG E₁, PG E₂, PG F₂α or PG F₃α produced no significant incorporation of radioactivity into the 19-hydroxy prostaglandin fractions. It is concluded that the biosynthesis of 19-OH PGs takes place via a separate pathway from that leading to the classical prostaglandins. Some observations on dynamic aspects of seminal prostaglandin production in man are presented, along with the results of a study on the species distribution of the 19-hydroxy E prostaglandins.

http://hdl.handle.net/1842/34196

The University of Edinburgh

Annexe Thesis Digitisation Project 2019 Block 22

Studies on seminal prostaglandins

Thesis or Dissertation

Doctoral

PhD Doctor of Philosophy