Gammoh, Noor

Wood, Andrew

Makar, Agata Natalia

2023-09-27T10:04:50Z

2023-09-27T10:04:50Z

2023-09-27

Autophagy is a cellular recycling mechanism that can be upregulated in cancer cells to provide energy and nutrient supply and promote cell survival. It has previously been reported that autophagy deficiency can result in tumour growth inhibition and enhanced sensitivity to chemotherapy suggesting that targeting autophagy is an attractive treatment option for cancer patients. To efficiently target autophagy as a treatment strategy, it is necessary to investigate the molecular autophagy pathway and develop novel potent inhibitors. Previous work in the lab, utilising proximity labelling assays and a genome-wide CRISPR-Cas9 screen have identified potential novel mechanisms by which autophagy can be regulated. The goals of my PhD project are to develop the necessary tools and characterise these new modulators in order to further our understanding of the regulation of autophagy in cells.

https://hdl.handle.net/1842/40959

http://dx.doi.org/10.7488/era/3710

en

The University of Edinburgh

Dudley, L.J., Cabodevilla, A.G., Makar, A.N., Sztacho, M., Michelberger, T., Marsh, J.A., Houston, D.R., Martens, S., Jiang, X., and Gammoh, N. (2019a). Intrinsic lipid binding activity of <scp>ATG</scp> 16L1 supports efficient membrane anchoring and autophagy. EMBO J. 38.

Dudley, L.J., Cabodevilla, A.G., Makar, A.N., Sztacho, M., Michelberger, T., Marsh, J.A., Houston, D.R., Martens, S., Jiang, X., and Gammoh, N. (2019b). Intrinsic lipid 165 List of references 165 binding activity of ATG 16L1 supports efficient membrane anchoring and autophagy . EMBO J.

Dudley, L.J., Makar, A.N., and Gammoh, N. (2020). Membrane targeting of core autophagy players during autophagosome biogenesis. FEBS J. 287, 4806–4821.

Regulating Autophagy

Autophagy

cancer cells

cell survival

autophagy deficiency

tumour growth inhibition

chemotherapy

sensitivity to chemotherapy

cancer patients

CRISPR-Cas9

Investigating potential new mechanisms of regulating autophagy

Thesis or Dissertation

Doctoral

PhD Doctor of Philosophy