Herpesvirus isolation from healthy sheep
dc.contributor.advisor
Scott, G. R.
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dc.contributor.author
Munro, Ranald
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dc.contributor.sponsor
Ministry of Overseas Development UK: Natural Resources Studentship
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dc.date.accessioned
2023-10-02T15:28:08Z
dc.date.available
2023-10-02T15:28:08Z
dc.date.issued
1975
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dc.description.abstract
One hundred and fifty healthy lambs were screened for herpesviruses. Nasal swabs were collected from fifty lambs, blood samples from fifty lambs and both nasal swabs and blood from a further fifty lambs. Virus isolation was attempted in foetal lamb kidney cell cultures. No virus was recovered.
A technique, involving hypotonic lysis of erythrocytes, was developed for the separation of ovine leucocytes. White blood cell recovery rates, percentage viability of leucocytes and the degree of red cell contamination compared favourably with other methods of leucocyte separation.
The virus isolation system was tested by propagating equid herpesvirus 2 in the foetal lamb kidney cells. Acridine orange staining of infected monolayers detected degenerative changes not readily discernible by haematoxylin and eosin staining methods.
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dc.identifier.uri
https://hdl.handle.net/1842/41013
dc.identifier.uri
http://dx.doi.org/10.7488/era/3752
dc.publisher
The University of Edinburgh
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dc.relation.isreferencedby
Y
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dc.subject
Annexe MSc Digitisation Project 2022 Block 36
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dc.title
Herpesvirus isolation from healthy sheep
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dc.title.alternative
A study of herpesvirus isolation from healthy sheep
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dc.type
Thesis or Dissertation
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dc.type.qualificationlevel
Masters
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dc.type.qualificationname
MSc Master of Science
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